- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
Data/Specifications
Immunogen:NITEGE synthetic peptide conjugate.
Clone: BC-13
Host: Mouse
Myeloma: x63-Ag8.653
Isotype: IgG1
Light Chain type: kappa
Specificity:Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated C-terminal neoepitope ...TEGE after cleavage between amino acids ..EGE373 and 374ARG.. within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).
Cross-reactivity:Human, Cow, Dog, Horse, Pig.
Purity: Affinity purified on protein G
Form: Liquid, 1 mL/vial
Concentration: 0.1 mg/mL
Storage: -20° C
Literature/Support
Aggrecan Antibody, clone BC-13 Insert (PDF, 334 KB)
Joint Disease and Aggrecan (blog post)
Ashinsky, B. G., Gullbrand, S. E., Bonnevie, E. D., Mandalapu, S. A., Wang, C., Elliott, D. M., ... & Smith, H. E. (2019). Multiscale and multimodal structure–function analysis of intervertebral disc degeneration in a rabbit model. Osteoarthritis and Cartilage, 27(12), 1860-1869.
How To Use
Applications
- Western Blot -Suggested dilution: 1:100.Detects a a band of approximately 60-70 kDa
- ELISA
- IHC
Technical Notes
Samples are usually deglycosylated using 0.01 Units chondroitinase ABC (Sigma), 0.01
Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG
of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry
(1, 2).
