- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Data/Specifications
Ig Isotype: Rat IgG1k
Specifcity:Keyhole limpet hemocyanin (KLH)
Form:The biotin conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3.
Storage:Store at 2 – 8 °C
Literature/Support
PDF Insert
How To Use
Applications:
- Used for Flow cytometry: working dilution ≤ 1 ug / 106 cells
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs
