- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Symbols/Related Terms:
- COL1A1
- COL1A2
- osteogenesis imperfecta
Data/Specifications
Reconstitution: Use 0.5 M acetic acid, pH 2.5 at 4°C. Collagen solubility 10 mg/ml. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.
Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.
Source: Goat tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation.
Form: lyophilized, 10 mg/vial
Purity: SDS-PAGE 90%
Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Literature/Support
Goat Type I Collagen Insert (PDF)
Note: inserts are for review only. Please use the insert that was sent with the product to ensure that the correct revision insert is being used for the product purchased.
How To Use
Key Applicatons:
- Used for goat collagen type I standard.
- Antigen for antibody production.
- Coating material for cell culture studies.
- Collagen microfibrils will activate platelets causing aggregation.
Note: may not be appropriate for 3D Gel formation
